Additional file 2 of circRIP2 accelerates bladder cancer progression via miR-1305/Tgf-β2/smad3 pathway

Additional file 2: Figure S2. Tgf-β2 inhibitor revered cancer promotive role of circRIP2 in bladder cancer cells in vitro. A,B. CCK8 assay was performed to detect cell viability of bladder cancer cells; C,D. Cell potential to replicate and self-renew was reflected by clone formation; E,F. Rate of wound healing assay showed cell potential of migration; scale bar: 100μm. G,H,I,J,K. Trans-well migration and matrigel invasion assay showed cell potential of migration and invasion of bladder cancer cells; scale bar: 25μm.

附加文件2：图S2。TGF-β2抑制剂（Tgf-β2 inhibitor）可逆转环状RNA RIP2（circRIP2）在体外膀胱癌细胞中的促癌作用。A、B：采用CCK8实验检测膀胱癌细胞的细胞活力；C、D：通过克隆形成实验反映细胞的增殖与自我更新潜能；E、F：划痕愈合实验的愈合速率体现细胞的迁移能力，标尺：100μm。G、H、I、J、K：采用Transwell迁移实验与基质胶侵袭实验检测膀胱癌细胞的迁移与侵袭潜能，标尺：25μm。