Dataset of live imaging of RNA Polymerase II CTD phosphorylation in zebrafish embryos

Images are recruited RNA polymerase II Serine 5 (Pol II Ser5P) and RNA polymerase II Serine 2 (Pol II Ser2P) in live sphere stage zebrafish embryos, visualized with antibody fragments (Fab) labelled with Janelia Fluor 647 and Alexa Fluor 488, respectively.<br> <br> Dataset "<strong>EmbryoC_20ms_040.nd2" </strong>is recorded by 20 ms exposure time and contains 120 frames. Dataset "<strong>EmbryoC_50ms_042.nd2</strong>" contains images recorded with 50 ms exposure time and contains 61 frames.本数据集的成像靶标为活体球体期斑马鱼胚胎中的RNA聚合酶II（RNA polymerase II）丝氨酸5位点磷酸化形式（Pol II Ser5P）与RNA聚合酶II丝氨酸2位点磷酸化形式（Pol II Ser2P），二者分别通过标记有Janelia Fluor 647与Alexa Fluor 488的抗体片段（Fab）完成可视化。 数据集<strong>EmbryoC_20ms_040.nd2</strong>采用20 ms曝光时长录制，共包含120帧图像；数据集<strong>EmbryoC_50ms_042.nd2</strong>采用50 ms曝光时长录制，共包含61帧图像。