Lubricin expression in human BM MSCs after pellet culture.
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(A) Schema for the study of in vitro chondrogenesis. BM MSCs were prepared from 5 donors. 2.5 × 105 human BM MSCs at passage 2 were pelleted and cultured in chondrogenic medium for 7 or 21 days. (B) Histological sections of cartilage pellets at day 21 stained with Safranin-O/Fast Green. (C) Cartilage pellets immunostained with lubricin. (D) Cartilage pellets immunostained without primary lubricin antibody as negative control. (E) Extent score for lubricin positive cell rate [37] (n = 5, *p
(A) 体外软骨生成研究的实验方案：从5名供体中分离制备骨髓间充质干细胞（Bone Marrow Mesenchymal Stem Cells, BM MSCs）。取第2代的2.5×10⁵个人骨髓间充质干细胞离心聚球，于软骨生成培养基中分别培养7天或21天。 (B) 第21天软骨球的组织学切片，经番红O/固绿（Safranin-O/Fast Green）染色。 (C) 采用润滑素（lubricin）进行免疫染色的软骨球样本。 (D) 以未添加润滑素一抗的组别作为阴性对照的软骨球免疫染色样本。 (E) 润滑素阳性细胞率的量化评分[37]（n=5，*p）
