Supplementary video S4: Extramural vascular supply to the male mouse urinary bladder

Cleared lower abdomen and pelvis of adult C57Bl/6 male mouse perfused, intracardially with a DY649 conjugate of Tomato lectin and viewed with light sheet microscopy (Ultramicroscope Blaze, Miltenyi Biotec, Germany). Segmentations of vessels (Syglass) and organs (Imaris) demonstrating the origins of the vasculature supply of the urinary bladder in the context of the entire pelvis. In this visualization the left internal iliac is not shown. Red = vessels of the external supply (abdominal aorta, common iliac, internal iliac, urogenital artery); orange = vesicular arteries; yellow = intramural vessels of the bladder, i.e., deep dorsal artery (DDA), deep ventral artery (DVA) and superficial ventrolateral artery (SVLA).

成年C57Bl/6雄性小鼠经心脏灌注番茄凝集素（Tomato lectin）DY649偶联物后，对其下腹部及盆腔组织进行透明化处理，采用光片显微镜（Ultramicroscope Blaze，德国美天旎生物科技公司）完成成像。通过Syglass软件对血管、Imaris软件对器官进行图像分割，以此展示完整盆腔背景下膀胱血管供应的起源位点。本可视化图像未显示左侧髂内动脉。其中红色代表外源性供血血管（腹主动脉、髂总动脉、髂内动脉、泌尿生殖动脉）；橙色代表膀胱动脉；黄色代表膀胱壁内血管，具体包括背深动脉（DDA）、腹深动脉（DVA）及腹外侧浅动脉（SVLA）。