CIL:836, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 68,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

RIGHT image of a stereo pair.

将兔腰大肌骨骼肌纤维维持于恒定长度，并通过浸入甘油诱导其进入肌僵直状态。随后将纤维置于Heuser型冷冻压机（Heuser-type cryopress）中，通过与液氦冷却铜块接触实现快速冷冻，再转移至Balzers 400型冷冻断裂仪中完成冷冻蚀刻操作。接着以24°角采用铂-碳（Pt-C）对样品进行旋转复型，随后在加速电压为100 keV的JEOL 100CX2型透射电子显微镜下进行观测。

以68000倍的放大倍率将图像记录于柯达4489胶片上。以+5°和-5°倾角拍摄立体图像对，随后使用尼康Coolscan 9000ED扫描仪以20微米的像素间距对胶片进行扫描。

本图为立体图像对的右侧图像。