Dorsal-GFP entry into nuclei in the absence of degron-tagged CactDN under the conditions of laser illumination used in this study.

A single embryo expressing Dorsal-GFP was imaged from nuclear cycle (nc) 12 until gastrulation (st.6), demonstrating the formation of a normal ventral-to-dorsal gradient of nuclear accumulation in the absence of CactDN expression. In brief, the embryo was imaged under three conditions as outlined in Fig 9A: (i) imaging was initiated at nc12 using the low power (3.1%) 488nm laser; (ii)10min later (nc13) high power (10%) 488nm was applied for a period of 20min; finally, after a resting period of 35min, imaging was reinitiated at low power (3.1%) 488nm, extending until gastrulation. The movie is a sequential compilation of these 3 imaging sessions taken over time, and due to lack of CactDN serves as a control for the movies of embryos expressing the various degron-tagged versions of CactDN. Snapshots from the movie are shown in Fig 9B–9B“‘. (ZIP)表达背蛋白-绿色荧光蛋白（Dorsal-GFP）的单个胚胎，于核周期（nuclear cycle，简称nc）12至原肠胚形成（gastrulation，简称st.6）阶段被成像，展现了未表达CactDN时细胞核内蛋白积累量形成正常腹-背侧梯度的过程。简言之，该胚胎按照图9A所述的三种条件完成成像：(i) 于nc12时采用低功率（3.1%）488nm激光启动成像；(ii) 10分钟后（对应nc13）切换至高功率（10%）488nm激光，持续成像20分钟；最终，在静置35分钟后，再次以低功率（3.1%）488nm激光重启成像，直至原肠胚形成阶段。本影片为上述三次成像会话的时序拼接产物，因未表达CactDN，可作为表达各类携带降解标签的CactDN变体胚胎成像影片的对照。该影片的快照展示于图9B–9B‴。（ZIP）