HSPC scRNA seq

We performed scRNA sequencing to understand Helios function in the heterogeneous hematopoietic stem and progenitor (HSPC) cells population. We combined this technology to CITE-sequencing to obtain measurement at the protein and transcriptome level and to HASH-sequencing in order to measure these data simultaneously in WT and Helios deficient HSPC in duplicate. Flow cytometry purified HSPC (Lin-Sca1+ckit+ cells) from 2 WT and 2 Helios deficient mice were stained for the cell surface proteins CD41, CD48, CD150 and Flt3 with oligonucleotide-tagged antibodies to perform CITE-sequencing. HSPC from each mouse were labelled with a unique oligonucleotide-tagged antibody anti-CD45 and anti-MHCI (HASH-sequencing) to identify them. Equal amount of HSPC (2500 cells) from each mouse were pooled and loaded to perform scRNA sequencing.