Electronic Supplementary Material (data)

The heart rate and CTmax data from all individuals used in this study. These data were collected from 25 different families of chinook salmon (5 females and 5 males mated in all combinations). Heart rates were counted from electrocardiograms and recorded along with the corresponding temperature. These data were then analyzed to find the Arrhenius break temperature of heart rate, which corresponds to a drop in the temperature sensitivity (Q10) of heart rate. The additive genetic, non-additive genetic, and maternal effects contributing to cardiac performance and CTmax were calculated by partitioning the variation in these phenotypes to Sire (i.e. father) and Dam (i.e. mother) ID and their interaction using a two-way ANOVA.

本研究所用全部受试个体的心率与临界高温(CTmax)数据集。此类数据采集自25个奇努克鲑鱼家系，该家系由5尾雌性与5尾雄性个体进行全组合交配构建获得。心率通过心电图记录获取，并同步记录对应温度。随后对该数据集开展分析，以确定心率的阿伦尼乌斯拐点温度，该温度对应心率的温度敏感性(Q10)出现下降。通过双向方差分析(two-way ANOVA)，将这些表型的变异按父本（即父亲）、母本（即母亲）的ID及其交互效应进行拆分，以此计算得到影响心脏功能与临界高温(CTmax)的加性遗传效应、非加性遗传效应以及母体效应。