SH1399372.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于界定、识别、传播以及处理基于DNA的物种假说（Species Hypotheses，以下简称SH）。国际核苷酸序列数据库中的所有真菌内部转录间隔区（Internal Transcribed Spacer，简称ITS）序列，均通过应用一系列动态距离阈值（0.5%至3.0%）被聚类至近似物种级别的集群。所有SH均会被赋予一个以数字对象标识符（Digital Object Identifier，简称DOI）为形式的唯一且稳定的名称，其分类学与生态学注释将通过分布式的网络第三方注释工作完成验证。所有SH都会尽可能结合其包含的全部序列的鉴定结果，关联至对应的分类单元名称及其所属的分类层级（如门、纲、目等）。将为每个SH选定一条自动或人工指定的序列作为代表序列。此类代表序列已对外发布（https://unite.ut.ee/repository.php），可供科研共同体用于本地序列相似性检索、新一代测序分析流程等场景。随着公共真菌ITS序列数量的持续增长，该系统及其配套数据将自动完成更新。