Dataset from Mouse 2 for Sit & Goard: Distributed and Retinotopically Asymmetric Processing of Coherent Motion in Mouse Visual Cortex

Accompanying dataset for Sit KK, Goard MJ. Distributed and Retinotopically Asymmetric Processing of Coherent Motion in Mouse Visual Cortex.<br><br>This dataset contains the data for mouse 2 / 10<br>Mouse SKKS058<br>See all data in collection here: https://doi.org/10.35092/yhjc.c.5018363<br>These datasets contain calcium imaging data taken from L2/3 of the primary visual cortex in mice. Mice were head fixed and passively watching a random dot kinematogram stimulus. The stimulus data vector for processing these data are also included. The accompanying code to analyze these data can be found at: https://github.com/kevinksit/CoherentMotionProject<br>Data are either in preprocessed DFF matrices, which represent the change in neural activity compared to a baseline or raw images from the microscope. All data are processed with MATLAB 2018b.<br>The purpose of these data is to understand the organization of neural activity in response to coherent motion in the murine visual cortex.<br>The manuscript associated with these data is Sit KK, Goard MJ. Distributed and Retinotopically Asymmetric Processing of Coherent Motion in Mouse Visual Cortex.<br>ABSTRACTPerception of visual motion is important for a range of ethological behaviors in mammals. In primates, specific visual cortical regions are specialized for processing of coherent visual motion. However, whether mouse visual cortex has a similar organization remains unclear, despite powerful genetic tools available for measuring population neural activity. Here, we use widefield and 2-photon calcium imaging of transgenic mice to measure mesoscale and cellular responses to coherent motion. Imaging of primary visual cortex (V1) and higher visual areas (HVAs) during presentation of natural movies and random dot kinematograms (RDKs) reveals varied responsiveness to coherent motion, with stronger responses in dorsal stream areas compared to ventral stream areas. Moreover, there is considerable anisotropy within visual areas, such that neurons representing the lower visual field are more responsive to coherent motion. These results indicate that processing of visual motion in mouse cortex is distributed heterogeneously both across and within visual areas.

本数据集为Sit KK与Goard MJ发表的《小鼠视觉皮层对连贯运动的分布式且视网膜拓扑不对称加工》一文的配套数据。

本数据集包含第2/10号实验小鼠SKKS058的相关实验数据。
可通过以下链接获取该数据集合集的全部内容：https://doi.org/10.35092/yhjc.c.5018363

本数据集包含取自小鼠初级视觉皮层L2/3层的钙成像数据。实验中小鼠头部固定，并被动观看随机点运动图（random dot kinematogram，RDK）刺激，同时附带用于数据分析的刺激数据向量文件。

用于分析本数据集的配套代码可通过以下链接获取：https://github.com/kevinksit/CoherentMotionProject

数据集内容分为两类：代表神经活动相对于基线变化量的ΔF/F（DFF）预处理矩阵，或是来自显微镜的原始图像；所有数据均通过MATLAB 2018b完成处理。

本数据集的研究目的为解析小鼠视觉皮层中响应连贯运动的神经活动组织模式。

与本数据集配套的学术论文为Sit KK、Goard MJ发表的《小鼠视觉皮层对连贯运动的分布式且视网膜拓扑不对称加工》。

**摘要**：视觉运动感知对哺乳动物的多种生态行为至关重要。在灵长类动物中，特定视觉皮层区域专门负责加工连贯视觉运动。尽管当前已有强大的遗传工具可用于记录群体神经活动，但小鼠视觉皮层是否存在类似的功能组织模式仍不明确。本研究通过对转基因小鼠进行宽场成像与双光子钙成像，分别记录中尺度与单细胞水平的连贯运动响应。在呈现自然电影与随机点运动图（RDK）刺激期间，对初级视觉皮层（V1）以及更高视觉皮层区域（HVAs）的成像结果显示，不同脑区对连贯运动的响应存在差异：背侧通路脑区的响应强于腹侧通路脑区。此外，单个视觉皮层内部存在显著的各向异性，即代表下视野的神经元对连贯运动的响应更强。上述结果表明，小鼠皮层中的视觉运动加工在跨视觉脑区以及单个脑区内部均呈现异质性分布式特征。