Supplemental Material for Pujari and Cullen, 2024

Signaling pathways control the response to stimuli. In yeast, a signaling (MAPK) pathway controls a fungal behavioral response called filamentous growth. A genetic screen was performed to identify spontaneous mutants that show hyperactivity of a MAPK pathway-dependent reporter. Select mutants were analyzed by whole-genome sequencing. New alleles in known regulatory proteins were identified, including an inhibitory domain in the C-terminus of the transcription factor Ste12p. Our results highlight the combinatorial effects of multiple positive and negative regulatory inputs in regulating a MAPK-dependent differentiation response.

信号通路可调控生物体对刺激的应答。在酵母中，一条丝裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）信号通路调控一种名为丝状生长的真菌行为应答。研究人员通过遗传筛选鉴定出了可使MAPK通路依赖性报告基因呈现高活性的自发突变体，随后对筛选获得的部分突变体开展全基因组测序分析。研究人员在已知调控蛋白中发现了新的等位基因，其中包括转录因子Ste12p羧基末端的一处抑制结构域。本研究结果凸显了多组正向与负向调控输入在调控MAPK依赖性分化应答中的组合效应。