Knockout of the B1 subunit of the GA-binding protein (GABP) [RNA-seq]

We knocked out different exons corresponding to different sections of GABP, a transcription factor known to bind to the telomerase reverse transcriptase (TERT) promoter with the G228 mutation. We investigated the effects of the knockouts in the regulation of TERT expression and other subunits of GABP. GABP genomic binding sites were determined through chromatin immunoprecipitation sequencing (ChIP-seq), and gene expression was determined through total RNA sequencing (RNA-seq). Combining data pertaining to GABP binding sites and gene expression provided insight into the molecular mechanisms of maintaining the length of telomeres in cancers. RNA-seq for 33 conditions. Eight of the conditions were knockouts of the B1 subunit of GABP from exon 2 to exon 9, with four of these conditions being in A-375 cells and four of these conditions being in UM-UC-3 cells. Thirteen of the conditions were knockouts of exon 2 of the B1 subunit, with eight of the conditions being in A-375 cells and five of these conditions being in UM-UC-3 cells. Two of the samples were knockouts of exon 9 of the B1 subunit in A-375 cells. Seven of the samples were control samples that knocked out ROSA26, with four of these conditions being in A-375 cells and three of these conditions being in UM-UC-3 cells. Two of the samples were also control samples with no knockouts. B1TKO: includes the combination of all samples consisting of knockouts of exons 2-9 in the B1 subunit of GABP (B1KO) and samples consisting of knockouts of exon 2 in the B1 subunit of GABP (B1X2KO).