Data for: Positive in situ hybridization for ZIKV RNA in MFI tissues and gravid uteri slides

Zika virus (ZIKV) can be transmitted vertically from mother to fetus
during pregnancy, resulting in a range of outcomes, including severe birth
defects and fetal/infant death. Potential pathways of vertical
transmission in utero have been proposed but remain undefined. Identifying
the timing and routes of vertical transmission of ZIKV may help us
identify when interventions would be most effective. Furthermore,
understanding what barriers ZIKV overcomes to effect vertical transmission
may help improve models for evaluating infection by other pathogens during
pregnancy. The images included in this dataset belong to a study that
aimed to determine the pathways of vertical transmission using a rhesus
macaque model. Pregnant rhesus macaques were inoculated with
104 plaque forming using (PFA) of an African-lineage ZIKV isolate
(ZIKV-DAK) at gestational day 30 (term is 165 days). Maternal-fetal
interface and gravid uteri were evaluated for ZIKV RNA using in situ
hybridization (ISH). The included images are the maternal fetal interface
tissues and coronal sections evaluated for ZIKV via ISH, areas were ZIKV
RNA was detected have pink/red staining. The pattern of infection observed
in the maternal-fetal interface provides evidence of vertical ZIKV
transmission through the fetal membranes.

寨卡病毒（Zika virus, ZIKV）可在妊娠期间经母婴垂直传播，引发一系列不良妊娠结局，包括严重出生缺陷及胎儿/新生儿死亡。尽管学界已提出若干宫内垂直传播的潜在路径，但具体机制仍未明确。明确寨卡病毒垂直传播的时机与途径，有助于确定干预措施的最佳实施窗口。此外，解析寨卡病毒突破母婴屏障以实现垂直传播的机制，或可助力优化妊娠期间其他病原体感染的评估模型。本数据集所附图像来自一项以恒河猴为模型探究寨卡病毒垂直传播路径的研究。研究人员于妊娠第30天（恒河猴正常孕期为165天）对妊娠恒河猴接种10⁴空斑形成单位（plaque forming unit, PFA）的非洲谱系寨卡病毒分离株ZIKV-DAK。通过原位杂交（in situ hybridization, ISH）技术检测母胎界面及妊娠子宫内的寨卡病毒RNA。本数据集包含的图像为经原位杂交检测寨卡病毒的母胎界面组织与冠状切面，其中寨卡病毒RNA阳性区域呈粉红色/红色染色。在母胎界面观察到的感染模式，为寨卡病毒通过胎膜实现垂直传播提供了实验证据。