Dataset for "Gap junctions desynchronize a neural circuit to stabilize insect flight"

<strong>Data guide</strong> For each figure, we provide one data folder. Each folder contains one overview Excel file that provides all source data that is shown in the respective figure. For some figure panels additional subfolders with original data files are provided. Please note that the data for figures 3A-J (except F and G) have been uploaded together with the analyses and simulation code and are accessible under the doi: 10.5281/zenodo.7740678 <strong>Data Figure 1: </strong> Overview Excel file with all source data: Figure 1.xlsx Folder Panel A: Recordings shown in A and G with sorted spikes: figure 1a_original recording.smr Data matrix of waveforms shown in A as text file: figure 1a_waveforms_200818_561.5-566.5sec.txt Folder Panels B+F: all original recordings with sorted spikes that have been analyzed for panels B and F: 171106_m1.SMR 181029_m1.smr 190522_m1.SMR 200305_m1.smr 200818_m1.smr 200824_m1.smr 200827_m1.SMR 201005_m1.smr Folder Panel C: all original recordings as .smr (Spike2) files that went into the analyses shown in figure 1c. 100 recordings in folders “Fly 1 to Fly 100” 1 recording in folder “single animal” the waveforms of these 101 animals are also as text in file Figure 1.xlsx Folder Panel D The original data file of the recording shown in figure 1D as axon file: 20128002_OUT1_firing.abf The selected waveforms shown in figure 1D are also as text in file Figure 1.xlsx All recordings that underlie the analysis in figure 1E are as number matrices in the file Figure 1.xlsx The spike events shown in figure 1G are as number matrices in the file Figure 1.xlsx <strong>Data Figure 2: </strong> Overview Excel file with all source data: Figure 2.xlsx The number matrices for all traces shown in figure 2A are as number matrices in the file Figure 2.xlsx Panel B: all number matrices of the event times that underlie the analyses shown in figure 2B: Folder “control”: .txt of event times of the 7 control animals Folder “ShakB-RNAi”: .txt of event times of the 7 ShakB-RNAi-kd animals Folder “ShakB-oe”: .txt of event times of the 7 ShakB-oe (overexpression) animals Panel C original confocal image stack for image data shown in figure 2C: original Leica file: figure 2c_image stack.lif Folder “tiff” with same image stack saved as tiff Panel D: The number matrices underlying the waveforms of the traces shown in Figure 2D are contained in the file Figure 2.xlsx Panel E: The number matrices underlying the waveforms of the traces shown in Figure 2E are contained in the file Figure 2.xlsx Panel F: The number matrices of all pre- and postsynaptic voltage areas of paired MN recordings and the analyses thereof for Figure 2F (left) are contained in the file Figure 2.xlsx. The number matrices of all pre- and postsynaptic voltage amplitudes of paired MN recordings and the analyses thereof for Figure 2F (right) are contained in the file Figure 2.xlsx Panel G: The number matrices underlying the waveforms of the traces shown in Figure 2G are contained in the file Figure 2.xlsx The number matrices underlying the waveforms of the traces shown in Figure 2H are contained in the file Figure 2.xlsx The number matrices underlying the waveforms of the traces shown in Figure 2I are contained in the file Figure 2.xlsx <strong>Data Figure 3: </strong>(all data for Fig. 3 except f and g are at doi: 10.5281/zenodo.7740678) Overview Excel file with all source data for Figures 3G and F: Figure 3.xlsx Panel F: The number matrices for all traces shown in figure 3F (command voltage +50mV) and for 14 additional command voltage steps are contained in the file Figure 3.xlsx Panel F: The number matrices for the waveforms of all traces shown in figure 3F (command voltage +50mV) and for 14 additional command voltage steps are contained in the file Figure 3.xlsx Panel G: The number matrices for the waveforms of all traces shown in figure 3G are contained in the file Figure 3.xlsx original data file from which the traces in figure 3G (top) were taken: control MN4_3_0s-87s.SMR original data file from which the traces in figure 3G (bottom) were taken: UAS shab81 2022_10_07_0s-87s.SMR <strong>Data Figure 4: </strong> Overview Excel file with all source data: Figure 4.xlsx Panel A: The number matrices for all event signals and all waveform traces shown in figure 4A are as number matrices in the file Figure 4.xlsx Figure 4B (top): The number matrices for the waveforms (voltage recording and fit) shown in Figure 4b (top) are contained in the file Figure 4.xlsx The number matrices for the waveforms of the voltage recordings that were fitted to obtain the averages shown in Figure 4B top are contained in the file Figure 4.xlsx The fitting parameters and results for Figure 4B (top) are contained in the file Figure 4.xlsx Figure 4B (middle): The number matrices for the waveforms (calcium imaging and fit) shown in Figure 4b (middle) are contained in the file Figure 4.xlsx The fitting parameters and results for Figure 4B (middle) are contained in the file Figure 4.xlsx The original imaging data for this analysis is contained in movie S4 Figure 4B (bottom): The number matrices for the waveforms (wingbeat frequency from events and fit) shown in Figure 4b (bottom) are contained in the file Figure 4.xlsx The fitting results are contained in the file Figure 4.xlsx Figure 4C: The number matrices for the waveform shown in Figure 4C are contained in the file Figure 4.xlsx The original calcium imaging movie as .avi file: DLM6_Imaging.avi The original calcium imaging movie as tiff stack: file DLM6_imaging_tiff The fitting results are contained in the file Figure 4.xlsx Figure 4E: The number matrices for the waveforms shown in Figure 4E are contained in the file Figure 4.xlsx The number matrices for the waveforms that resulted in the average waveforms shown in Figure 4E are contained in the file Figure 4.xlsx The original data files for analyses in Figure 4E are contained as text files in the folders ShakB_oe (7 text files) and control (7 text files)**数据说明** 每个图片对应一个独立的数据文件夹。每个文件夹内均包含一份总览Excel文件，内含对应图片展示的全部原始源数据。部分图片面板还附带了包含原始数据文件的额外子文件夹。请注意，图3A-J（F、G除外）的相关数据已随分析与仿真代码一同上传，可通过DOI：10.5281/zenodo.7740678获取。 **图1数据集** 总览Excel文件（包含全部源数据）：Figure 1.xlsx ### 面板A文件夹 用于展示面板A与G的电生理记录文件，内含已分选的峰电位：figure 1a_original recording.smr 面板A展示的波形数据矩阵（文本文件格式）：figure 1a_waveforms_200818_561.5-566.5sec.txt ### 面板B+F文件夹 用于面板B与F分析的全部带已分选峰电位的原始记录文件： 171106_m1.SMR、181029_m1.smr、190522_m1.SMR、200305_m1.smr、200818_m1.smr、200824_m1.smr、200827_m1.SMR、201005_m1.smr ### 面板C文件夹 用于图1c分析的全部原始记录文件，格式为.smr (Spike2) 文件。 包含100份记录，存于「Fly 1 to Fly 100」文件夹中；另有1份记录存于「single animal」文件夹中。 该101份样本的波形数据同样以文本形式存储于文件Figure 1.xlsx内。 ### 面板D文件夹 用于图1D展示的记录的原始数据文件（Axon格式）：20128002_OUT1_firing.abf 图1D中选取的波形数据同样以文本形式存储于文件Figure 1.xlsx内。 用于图1E分析的全部记录数据均以数值矩阵形式存储于文件Figure 1.xlsx内。 图1G展示的峰电位事件数据均以数值矩阵形式存储于文件Figure 1.xlsx内。 **图2数据集** 总览Excel文件（包含全部源数据）：Figure 2.xlsx 图2A展示的所有轨迹的数值矩阵均存储于文件Figure 2.xlsx内。 ### 面板B 用于图2B分析的全部事件时间数值矩阵： - 「control」文件夹：7只对照动物的事件时间文本文件 - 「ShakB-RNAi」文件夹：7只ShakB-RNAi敲低动物的事件时间文本文件 - 「ShakB-oe」文件夹：7只ShakB过表达动物的事件时间文本文件 ### 面板C 用于图2C展示的共聚焦图像栈的原始数据文件（Leica格式）：figure 2c_image stack.lif 「tiff」文件夹：已转换为TIFF格式的同款图像栈文件 ### 面板D 图2D展示的轨迹波形对应的数值矩阵均存储于文件Figure 2.xlsx内。 ### 面板E 图2E展示的轨迹波形对应的数值矩阵均存储于文件Figure 2.xlsx内。 ### 面板F 用于图2F左侧（成对运动神经元记录的突触前、突触后电压面积及其分析结果）的数值矩阵均存储于文件Figure 2.xlsx内。 用于图2F右侧（成对运动神经元记录的突触前、突触后电压振幅及其分析结果）的数值矩阵均存储于文件Figure 2.xlsx内。 ### 面板G 图2G展示的轨迹波形对应的数值矩阵均存储于文件Figure 2.xlsx内。 ### 面板H 图2H展示的轨迹波形对应的数值矩阵均存储于文件Figure 2.xlsx内。 ### 面板I 图2I展示的轨迹波形对应的数值矩阵均存储于文件Figure 2.xlsx内。 **图3数据集** （图3除F、G外的全部数据可通过DOI：10.5281/zenodo.7740678获取） 用于图3F与3G的总览Excel文件（包含全部源数据）：Figure 3.xlsx ### 面板F 图3F（指令电压+50mV）的所有轨迹及额外14组指令电压阶跃对应的数值矩阵均存储于文件Figure 3.xlsx内。 ### 面板F 图3F（指令电压+50mV）的所有轨迹波形对应的数值矩阵及额外14组指令电压阶跃数据均存储于文件Figure 3.xlsx内。 ### 面板G 图3G展示的所有轨迹波形对应的数值矩阵均存储于文件Figure 3.xlsx内。 图3G上方轨迹的原始数据文件：control MN4_3_0s-87s.SMR 图3G下方轨迹的原始数据文件：UAS shab81 2022_10_07_0s-87s.SMR **图4数据集** 总览Excel文件（包含全部源数据）：Figure 4.xlsx ### 面板A 图4A展示的所有事件信号与波形轨迹对应的数值矩阵均存储于文件Figure 4.xlsx内。 ### 图4B（上） 图4B（上）展示的波形（电压记录与拟合曲线）对应的数值矩阵均存储于文件Figure 4.xlsx内。 用于生成图4B（上）平均波形的电压记录波形数据均存储于文件Figure 4.xlsx内。 图4B（上）的拟合参数与结果均存储于文件Figure 4.xlsx内。 ### 图4B（中） 图4B（中）展示的波形（钙成像数据与拟合曲线）对应的数值矩阵均存储于文件Figure 4.xlsx内。 图4B（中）的拟合参数与结果均存储于文件Figure 4.xlsx内。 本次分析的原始成像数据包含于补充视频S4中。 ### 图4B（下） 图4B（下）展示的波形（事件信号与拟合曲线对应的翼搏频率）对应的数值矩阵均存储于文件Figure 4.xlsx内。 拟合结果均存储于文件Figure 4.xlsx内。 ### 图4C 图4C展示的波形对应的数值矩阵均存储于文件Figure 4.xlsx内。 原始钙成像视频（AVI格式）：DLM6_Imaging.avi 原始钙成像视频（TIFF栈格式）：DLM6_imaging_tiff 拟合结果均存储于文件Figure 4.xlsx内。 ### 图4E 图4E展示的波形对应的数值矩阵均存储于文件Figure 4.xlsx内。 用于生成图4E平均波形的波形数据均存储于文件Figure 4.xlsx内。 图4E分析所用的原始数据文件以文本形式存储于「ShakB_oe」（7个文本文件）与「control」（7个文本文件）文件夹中。