Solanum lycopersicum and BC204 RNAseq data

Plant biostimulants have been earmarked as one of the major groups of new plant growth promoting substances to drive a much-needed revolution in agriculture. One such PB, BC204, has been used to great success, but there is no peer-reviewed data to explain the possible mechanisms by which it exerts its effects. In this study, an RNA-seq approach was adopted to elucidate the effects of BC204 on shoot tissues of hydroponically grown <i>Solanum lycopersicum </i>seedlings at the molecular level. BC204, applied via foliar spray at a concentration of 0.05% (v/v), stimulated root and shoot biomass production, root and shoot length, and stem width compared to the untreated control plants. Out of the 33308 transcripts analysed, 18.059% of all genes were significantly differentially expressed between the control and treated groups, of which 8.776% were upregulated and 9.283% downregulated. Most notably, genes involved in signalling, stress and protein metabolism were upregulated, which could explain the observed increases in growth. Additionally, hormone metabolism and genes involved in transcription and other regulation processes were also upregulated. Genes involved in protein metabolism were mostly downregulated.

植物生物刺激素（Plant biostimulants）已被列为推动农业亟需变革的新型植物生长促进物质的核心类别之一。此类植物生物刺激素中的BC204已被成功应用，但目前尚无经过同行评议的研究数据阐明其发挥作用的潜在分子机制。本研究采用RNA测序（RNA-seq）技术，从分子层面解析BC204对水培培养的番茄（*Solanum lycopersicum*）幼苗地上组织的影响。本研究以0.05%（体积比）的浓度通过叶面喷施施加BC204，与未处理的对照组相比，该处理可显著促进番茄幼苗的根、地上部生物量积累，增加根长、地上部长度及茎粗。在分析的33308条转录本中，对照组与处理组间共有18.059%的基因发生显著差异表达，其中8.776%为上调表达，9.283%为下调表达。尤为关键的是，参与信号传导、胁迫响应及蛋白质代谢的基因呈现上调表达，这可解释观测到的生长提升现象。此外，激素代谢相关基因以及参与转录调控与其他调控过程的基因同样出现上调。而参与蛋白质代谢的基因则大多呈现下调表达。