BODIPY® FL Histamine as a new modality for quantitative detection of histamine receptor upregulation upon IgE sensitization in murine bone marrow-derived mast cells
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The purpose of the experiments presented was to test whether histamine receptors can be analyzed by staining with BODIPY® FL Histamine, a fluorescent ligand, using flow cytometry. Conclusion: BODIPY® FL Histamine was found to stain H4 transfected cells and mast cells in a concentration-dependent manner; this staining was competitively inhibited with unlabeled ligands. Furthermore, IgE sensitization was found to increase H4 receptor expression in mast cells. The cell phenotype (CF117, FcεRI) and immunocytostaining controls are described in the Materials and Methods.
创建时间：
2014-09-01
