Western blot results of different lectin reactions with different allergens.

+++: strong reaction, ++: moderate reaction, +: mild reaction, −: no reaction. Sugar binding specificities of lectin used for glycosylation analysis are as follows: GNA recognises terminal mannose (1–3), (1–2) and (1–6) linked to Mannose; DSA, recognises galactose (1–4) linked to N-acetylgalactosamine; MAA recognises sialic acid linked (2–3) to galactose; PNA recognises core galactose (1–3) N acetylgalactosamine; SNA, recognises sialic acid linked (2–6) to galactose; Anti 1, 3 Fucose recognises core (1,3) fucose. DSA, Datura stramonium agglutinin; GNA, Galanthus nivalis agglutinin; MAA, Maackia amurensis agglutinin; PNA, peanut agglutinin; SNA, Sambucus nigra agglutinin.+++: 强反应，++: 中度反应，+: 轻度反应，−: 无反应。用于糖基化分析的凝集素的糖结合特异性如下：雪花莲凝集素（Galanthus nivalis agglutinin, GNA）可识别以(1→3)、(1→2)及(1→6)糖苷键与甘露糖相连的末端甘露糖；曼陀罗凝集素（Datura stramonium agglutinin, DSA）可识别以(1→4)糖苷键与N-乙酰半乳糖胺相连的半乳糖；蒙古槐凝集素（Maackia amurensis agglutinin, MAA）可识别以(2→3)糖苷键与半乳糖相连的唾液酸；花生凝集素（peanut agglutinin, PNA）可识别以(1→3)糖苷键与N-乙酰半乳糖胺相连的核心半乳糖；黑接骨木凝集素（Sambucus nigra agglutinin, SNA）可识别以(2→6)糖苷键与半乳糖相连的唾液酸；抗1,3-岩藻糖可识别以(1,3)糖苷键相连的核心岩藻糖。各缩写对应全称如下：DSA为曼陀罗凝集素，GNA为雪花莲凝集素，MAA为蒙古槐凝集素，PNA为花生凝集素，SNA为黑接骨木凝集素。