The collected data of the PhD Thesis of Richards, Nicholas John 2025

This is a dataset of the data generated over the course of the PhD studies of Nicholas John Richards (2020-2024). It includes methods, analysis, figures, and example representative images. Much of the data deposited here are the microscope images in the form of TIFF files alongside the Excel sheets and GraphPad Prizm files that record, statistically analyse, and graph the data involved in the production of the figures of the above-mentioned thesis and published paper which resulted. The figures also included contained in PowerPoints. It is arranged by thesis chapter and experiment or stain performed. The complete methods used are also included in Word documents in a folder named as such.

The thesis by Nicholas John Richards to which this data relates is entitled: “The effects of the venom of the Tarantula spiders Poecilotheria regalis and Thrixopelma pruriens on skeletal muscle damage and nociception.” One paper has been published, this paper is Richards et al (2023), Indian Ornamental Tarantula (Poecilotheria regalis) Venom Affects Myoblast Function and Causes Skeletal Muscle Damage.

It was contributed to by Pradeep Vijaya Kumar (University of Reading, https://orcid.org/0000-0002-9894-2201) who collected the biochemistry data relating to the P. regalis venom (fluorescent PLA2, serine protease and metalloprotease assays) but these were analysed and graphed by Nicholas John Richards. The caseinolytic assays and biochemical assays on T. pruriens venom were performed by Nicholas John Richards.
Adedoyin Balogun (Hull York Medical School) also contributed by taking the images of the F4/80 staining of macrophages, these appeared as supplementary figures. These assay data and images were also later included in my thesis, with credit given, so are also included here. The copyright for the F4/80 images remains with Adedoyin Balogun. In addition, a further, at this time (March 2025) untitled, short communication is being drafted on the topic of nociception caused by the P. regalis venom.

Some data about an unrelated snake venom is the work of Sodiq Adeyemi (SA) (University of Reading, https://orcid.org/0000-0002-7664-7340). It is included because we used the same plate to investigate the caseinolytic activity of the P. regalis and the Bitis arietans Puff Adder snake. To avoid edits to the files and changes to the date modified this is also included.

本数据集涵盖尼古拉斯·约翰·理查兹（Nicholas John Richards）2020-2024年博士研究期间产生的全部相关数据，包含研究方法、分析结果、图表及典型示例图像。本库所存储的大部分数据为TIFF格式的显微镜图像，同时配套用于记录、统计分析及绘图的Excel表格与GraphPad Prism文件——该类文件用于生成前述博士论文及后续发表论文的配套图表。此外，部分图表以PowerPoint演示文稿形式收录。本数据集按博士论文章节及开展的实验或染色方式进行分类整理。完整的研究方法已整理至同名文件夹内的Word文档中。

本数据集关联的尼古拉斯·约翰·理查兹博士论文标题为：《捕鸟蛛Poecilotheria regalis与Thrixopelma pruriens毒液对骨骼肌损伤及伤害性感受的影响》。本研究已发表一篇学术论文：Richards等人（2023）的《印度观赏捕鸟蛛（Poecilotheria regalis）毒液影响成肌细胞功能并引发骨骼肌损伤》。

该数据集由雷丁大学的普拉迪普·维贾亚·库马尔（Pradeep Vijaya Kumar，ORCID：https://orcid.org/0000-0002-9894-2201）协助完成，其采集了与P. regalis毒液相关的生物化学数据（包括荧光磷脂酶A2（PLA2）、丝氨酸蛋白酶及金属蛋白酶检测实验），后续数据分析与绘图工作由尼古拉斯·约翰·理查兹完成。针对T. pruriens毒液的酪蛋白水解实验与生物化学检测均由尼古拉斯·约翰·理查兹独立开展。

赫尔约克医学院的阿德多延·巴洛贡（Adedoyin Balogun）贡献了巨噬细胞F4/80染色的成像结果，该部分内容作为补充图表呈现。上述检测数据与图像后续已在标注贡献者的前提下纳入本博士论文，因此同样收录于本数据集。F4/80染色图像的版权归阿德多延·巴洛贡所有。此外，目前（2025年3月）正在撰写一篇关于P. regalis毒液引发伤害性反应的未命名短篇通讯论文。

部分关于无关蛇毒的数据由雷丁大学的索迪克·阿德耶米（Sodiq Adeyemi，简称SA，ORCID：https://orcid.org/0000-0002-7664-7340）提供。该部分数据被收录的原因是，我们使用同一微孔板开展了P. regalis毒液与鼓腹咝蝰（Bitis arietans）毒液的酪蛋白水解活性检测。为保留原始文件的未编辑状态与修改时间戳，该部分数据一并纳入本数据集。