Highly sensitive profiling somatic mutations of thyroid cancer by nucleotide-enrichment-based MALDI-TOF MS assay

A novel multiple mutation technology that integrates nucleotide enrichment (NE)-assisted specific identification of variant alleles with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS), termedNE-MS, enabling the simultaneous identification of 26 thyroid cancer somatic hotspot mutations.一种整合了核苷酸富集（nucleotide enrichment, NE）辅助的变异等位基因特异性识别技术与基质辅助激光解吸电离飞行时间质谱（matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF MS）的新型多重突变检测技术，该技术被命名为NE-MS，可同时实现26种甲状腺癌体细胞热点突变的鉴定。