The role of m6A methylation modification in CPB2 toxin treatment of porcine small intestinal epithelial cells

In this experiment, m6A-seq sequencing technology was used to study the functional role of methylated molecules in the process of CPB2 processing porcine small intestinal epithelial cells. In this experiment, an IP library and an input library were built together. The IP library was enriched with m6A specific antibodies to generate methylated RNA, and the influence of m6A methylation modification on its expression was analyzed by bioinformatics. We finally concluded that m6A methylation modification may play a very important role after CPB2 toxin treats small intestinal epithelial cells. Overall design: Take the blank treatment as the control, and treat the porcine small intestinal epithelial cells with 20ug/ml CPB2 as the CPB2 group. The two groups simultaneously treat the small intestinal epithelial cells for 24h