VTA and NAC labeled ribosome from mPFC. Mus musculus
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下载链接:
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA393851
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资源简介:
Projection-dependent ribosome profling from mouse mPFC. Ribosome from NAC- and VTA- projecting mPFC cells were immunoprecipited using GFP-trap (Chromotech). Translating mRNA was isolated and analyzed Overall design: Cre-dependent ribosome TRAP mice were injected with CAV2-Cre in NAC and VTA. Projection-dependent ribosome labeling was enabled by isolating mPFC. Ribosome was genetically tagged with GFP in the activated cells and could be immunologically purified.
创建时间:
2017-07-11



