Additional file 1: of Design and application of a novel two-amplicon approach for defining eukaryotic microbiota

Table S1. Accuracy of taxonomic assignments of 18S and 28S rRNA variable genetic regions. Related to Fig. 1. Table S2. Primer characteristics and TestProbe3.0 results. Related to Fig. 2. Table S3. Amplicon sequencing statistics. Table S4. Read counts for eukaryotic microbes detected by two amplicon methods in patients suffering from SAM. Related to Fig. 3. Table S5. Genome and gene accessions used for comparisons with stool sample DNA and phylogenetic trees. Table S6. Differential abundance analysis of bacteria based on of Blastocystis carriage, using ALDEx2. Related to Fig. 5. Table S7. Association between eukaryote carriage or HIV reactivity and bacterial diversity. Related to Fig. 5. Table S8. Association between eukaryote abundance and clinical characteristics of patients hospitalized for SAM. (XLS 162 kb)

补充表S1：18S与28S 核糖体RNA（ribosomal RNA, rRNA）可变遗传区域的分类学分配准确性，关联图1。
补充表S2：引物特征及TestProbe3.0检测结果，关联图2。
补充表S3：扩增子测序统计数据。
补充表S4：两种扩增子方法在罹患SAM的患者中检测到的真核微生物读长计数，关联图3。
补充表S5：用于与粪便样本DNA及系统发育树（phylogenetic tree）进行比对的基因组与基因登录号。
补充表S6：基于芽囊原虫（Blastocystis）定植情况的细菌差异丰度分析，采用ALDEx2工具，关联图5。
补充表S7：真核生物定植或人类免疫缺陷病毒（HIV）反应性与细菌多样性之间的关联，关联图5。
补充表S8：真核生物丰度与因SAM住院的患者的临床特征之间的关联。（XLS 162 kb）