HBc interactome analysis using MS-based quantitative proteomics.
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https://figshare.com/articles/dataset/HBc_interactome_analysis_using_MS-based_quantitative_proteomics_/13231518
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ST-HBc-associated proteins were identified using MS-based proteomics. For this, cell lysate expressing ST-HBc or untagged HBc were treated or not with Benzonase before purification by affinity using Strep-Tactin. Eluted proteins were digested with trypsin and the resulting peptides submitted to MS-based proteomic analysis. The proteins were then identified and quantified in each sample before statistical analysis allowing to sort out proteins enriched with ST-HBc compared to untagged HBc used as negative control.
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创建时间:
2020-11-12



