Transcription profiling of mouse beta cells lacking NeuroD

The goal: Investigate NeuroD function in the mature beta cells. The model: Cross RIP-Cre to NeuroD flox/flox mouse to delete neuroD when insulin gene is activated; The methods: Islets are purified and RNA is extracted to see how the profile of gene expression is altered after neuroD is deleted from the beta cells. The comparison: Compare control with mutant; The hypothesis: Since neuroD is deleted, I would expect that neuroD mRNA will be decreased in the mutant samples; The samples: two types of samples will be submitted including control and conditional knockout (mutant); Flox/+,Ins (F/+,I) is the control and labled as group B; and Flox/-,Ins (F/-,I) is the conditional knockout and labled as group A