Transcriptomic Signatures of Kidney Injury in Human Renal Biopsy Specimens. Transcriptomic Signatures of Kidney Injury in Human Renal Biopsy Specimens

The pathogenesis and molecular signature of human acute kidney injury (AKI) remain incompletely understood, in part, owing to the heterogeneity of the disease. Further, the link between animal models and human AKI is not well characterized. Transcript expression of AKI in 39 native human renal biopsy samples was compared to 9 reference nephrectomies. These data demonstrate that the molecular signature segregates patients with AKI in spite of similar pathological and clinical phenotyping. Such molecular characterization will offer advantages in categorizing and understanding the underlying pathogenesis of phenotypically complex and heterogeneous forms of AKI. Overall design: Bulk 20 um thickness specimens from cross-sectional human kidney biopsies or nephrectomies embedded in OCT underwent RNA sequencing. All biopsy subjects had AKI. Sequencing performed on an Illumina HiSeq 4000.人类急性肾损伤（acute kidney injury，AKI）的发病机制与分子特征仍未完全阐明，部分原因在于该疾病的异质性。此外，动物模型与人类急性肾损伤之间的关联尚未得到充分阐释。本研究对39例人类自体肾活检样本中的急性肾损伤转录组表达水平，与9例对照肾切除术标本进行了对比分析。研究结果表明，尽管病理表型与临床表型均相似，但分子特征可有效区分急性肾损伤患者亚群。此类分子特征鉴定将为表型复杂且异质性显著的急性肾损伤亚型的分类及潜在发病机制解析提供助力。实验设计概况：取自横断面人类肾活检或肾切除术标本的20微米厚度批量样本，经OCT包埋后进行RNA测序。所有活检受试者均确诊为急性肾损伤。测序工作在Illumina HiSeq 4000测序平台上完成。