Immunofluorescence Images of iPSC-Derived Human Colonic Organoids (HCOs) Infected with Ebola and Marburg Viruses (Figure S1 Dataset)

Immunofluorescence images of iPSC-derived human colonic organoids (HCOs) that were either mock-infected or infected with Ebola virus (EBOV) or Marburg virus (MARV) at a multiplicity of infection (MOI) of 10. Samples were collected at 1 and 3 days post-infection (dpi). These images correspond to Figure S1 in the associated manuscript and represent a different iPSC-derived donor. Fluorescent markers include Mucin 2 (MUC2) or Villin 1 (VIL1) (green) for gut epithelial identity, EBOV NP or MARV NP/NC (red) for viral nucleoprotein detection, and DAPI (blue) for nuclear staining. Images were acquired at 10x and 20x magnification for overall visualization and at 63x magnification for detailed imaging of viral inclusion bodies. The dataset includes .tif files (some merged and some individual fluorescence channels used in the published figure panels), .czi files (multi-channel and z-stack raw microscopy data), and .gif files representing animated z-stack projections.本数据集包含诱导多能干细胞（induced pluripotent stem cell, iPSC）来源的人类结肠类器官（human colonic organoids, HCOs）的免疫荧光图像，样本分为模拟感染组，以及以感染复数（multiplicity of infection, MOI）为10分别感染埃博拉病毒（Ebola virus, EBOV）或马尔堡病毒（Marburg virus, MARV）的感染组。实验分别于感染后天数（days post-infection, dpi）1天和3天收集样本。该图像对应关联论文中的补充图S1，且来自不同的iPSC来源供体。 荧光标记方案如下：以绿色荧光标记黏蛋白2（Mucin 2, MUC2）或绒毛蛋白1（Villin 1, VIL1）以标识肠道上皮细胞身份；以红色荧光标记埃博拉病毒核蛋白（EBOV NP）或马尔堡病毒核蛋白/核衣壳（MARV NP/NC）以检测病毒核蛋白；以DAPI标记细胞核（蓝色荧光）。图像采集分为两类：以10倍和20倍物镜进行整体可视化成像，以63倍物镜对病毒包涵体进行细节成像。 本数据集包含三类文件：.tif格式文件（部分为多通道合并图像，部分为已发表图版中使用的单荧光通道图像）、.czi格式文件（多通道及Z-stack原始显微成像数据），以及代表动态Z-stack投影的.gif格式动画文件。