DNA metabarcoding reveals a broad dietary range for Tasmanian devils introduced to a naïve ecosystem

Top carnivores are essential for maintaining ecosystem stability and biodiversity. Yet, carnivores are declining globally and current in situ threat mitigations cannot halt population declines. As such, translocations of carnivores to historic sites or those outside the species’ native range are becoming increasingly common. As carnivores are likely to impact herbivore and small predator populations, understanding how carnivores interact within an ecosystem following translocation is necessary to inform potential remedial management and future translocations. Dietary analyses provide a preliminary assessment of the direct influence of translocated carnivores on a recipient ecosystem. We used a metabarcoding approach to quantify the diet of Tasmanian devils introduced to Maria Island, Tasmania, a site outside the species’ native range. We extracted DNA from 96 scats and used a universal primer set targeting the vertebrate 12S rRNA gene to identify diet items. Tasmanian devils on Maria Is..., , , # DNA metabarcoding reveals a broad dietary range for Tasmanian devils introduced to a naïve ecosystem

[https://doi.org/10.5061/dryad.2v6wwpzr1](https://doi.org/10.5061/dryad.2v6wwpzr1)

## Description of the data and file structure

This upload contains the fastq files generated via amplification with a 12Sv5 vertebrate primer pair and sequenced on a NextSeq 500 Sequencing system (Illumina, CA) and associated metadata used to identify the diets of Tasmanian devils on Maria Island, Tasmania.

Animals are anonymised with replicated denoted as \"R\". Each sample is indicated as coming from a known Tasmanian devil or collected from the ground and in which season the sample was collected is provided.

## Code/Software

Analyse this data using OBITools and the filtering parameters listed in the Materials and Methods section of the associated article.

顶级食肉动物对于维持生态系统稳定性与生物多样性至关重要。然而全球范围内食肉动物种群持续衰退，当前的原位威胁减缓措施无法阻止其种群下滑。有鉴于此，将食肉动物转移至其历史分布区域或原生分布范围以外的地点的做法正愈发普遍。由于食肉动物可能会对草食动物与小型捕食者种群造成影响，明确其在转移后如何在生态系统中开展相互作用，可为潜在的修复管理及后续的转移计划提供科学依据。饮食分析可初步评估被转移的食肉动物对接收生态系统的直接影响。本研究采用宏条形码（metabarcoding）技术方法，对引入至塔斯马尼亚州玛丽亚岛（该区域不属于袋獾（Tasmanian devil）原生分布范围）的袋獾的饮食进行定量分析。我们从96份粪便样本中提取DNA，并使用靶向脊椎动物12S rRNA基因的通用引物组鉴定饮食组分。玛丽亚岛上的袋獾……
# DNA宏条形码（DNA metabarcoding）技术揭示引入至未接触该物种的生态系统中的袋獾具有广泛饮食谱
https://doi.org/10.5061/dryad.2v6wwpzr1

## 数据与文件结构说明
本上传数据集包含通过12Sv5脊椎动物引物对扩增、在NextSeq 500测序系统（Illumina, 加利福尼亚州）上测序得到的fastq文件，以及用于鉴定塔斯马尼亚州玛丽亚岛袋獾饮食的相关元数据。
所有个体均已匿名，重复样本标记为"R"。每份样本均标注其来源为已知袋獾或是从地面采集，并附带样本的采集季节信息。

## 代码与软件
可使用OBITools工具包及相关论文材料与方法中列出的过滤参数对本数据进行分析。