Oxidative Verdoheme Formation and Stabilization by Axial Isocyanide Ligation

The effect of isocyanides as axial ligands on the formation and stability of verdoheme by oxidation has been
examined. The reaction of {FeIII(OEPO)}2 with t-butyl isocyanide under dioxygen-free conditions results in the
formation of (t-BuNC)2FeII(OEPO•) with an electron paramagnetic resonance at g = 2.009 with a peak-to-peak
separation of 23.5 G at 4 K. (OEPO is the trianion of octaethyloxophlorin and OEPO• is the radical dianion obtained
from OEPO by one-electron oxidation.) Exposure of chloroform solutions of either (2,6-xylylNC)2FeII(OEPO•) or
(t-BuNC)2FeII(OEPO•) to dioxygen followed by the addition of ammonium hexafluorophosphate results in their
transformation into the diamagnetic verdohemes, [(2,6-xylylNC)2FeII(OEOP)](PF6) and [(t-BuNC)2FeII(OEOP)](PF6),
yields 68 and 70%, respectively. (OEOP is the anion of octaethyl-5-oxaporphyrin.) The oxidation reactions of (2,6-xylylNC)2FeII(OEPO•) and (t-BuNC)2FeII(OEPO•) have also been monitored by 1H NMR spectroscopy. No resonances
due to paramagnetic products could be detected, the reactions appear to result only in the formation of the diamagnetic
verdohemes, and the products are not susceptible to further oxidation.