Differential expression of vascular endothelial growth factor () and platelet-derived growth factor () family genes in CD11b/Gr-1osteoclast precursors (OCPs) from tumor necrosis factor-transgenic (TNF-Tg) and wild-type (WT) mice

<b>Copyright information:</b>Taken from "Increased lymphangiogenesis in joints of mice with inflammatory arthritis"http://arthritis-research.com/content/9/6/R118Arthritis Research &amp; Therapy 2007;9(6):R118-R118.Published online 12 Nov 2007PMCID:PMC2246237. CD11b/Gr-1cells from peripheral blood and bone marrow from TNF-Tg (7 mice per array) and WT (23 mice per array) mice were pooled and purified by flow sorting. The RNA samples were subjected to microarray analysis using the GeneChip mouse genome 430A 2.0 array from Affymetrix. The array data on angiogenic gene expression were analyzed using GeneTraffic software. The expression ratio was calculated by dividing the mean value of the intensity of RNA signals from two separate arrays of TNF-Tg OCPs by that from WT cells.

<b>版权信息：</b>本文摘录自《Increased lymphangiogenesis in joints of mice with inflammatory arthritis》，原文链接：http://arthritis-research.com/content/9/6/R118，刊载于《Arthritis Research & Therapy》2007年第9卷第6期：R118-R118，于2007年11月12日在线发表，PMCID编号：PMC2246237。从外周血获取的CD11b/Gr-1细胞，以及肿瘤坏死因子转基因（TNF-Tg）小鼠（每组芯片阵列使用7只小鼠）与野生型（WT）小鼠（每组芯片阵列使用23只小鼠）的骨髓样本经混合后，通过流式分选完成纯化。提取的RNA样本采用昂飞（Affymetrix）公司的GeneChip小鼠基因组430A 2.0芯片阵列进行微阵列分析。针对血管生成相关基因表达的芯片数据，使用GeneTraffic软件进行分析。表达比值通过将两组独立的TNF-Tg破骨细胞前体细胞（Osteoclast Precursors, OCPs）的芯片阵列的RNA信号强度均值，除以野生型细胞的对应信号强度均值计算得到。