Protective effects of alpha-lipoic acid on diazinon-induced renal toxicity in rats: an immunohistochemistry study

The current study aimed to examine the impacts of alpha-lipoic acid (ALA) on oxidative stress parameters and the expression of fibronectin and laminin in the renal corpuscle of male Wistar rats following exposure to diazinon (DZN). The animals were distributed into the following groups: control, DZN (40 mg/kg), DZN + ALA (40 mg/kg + 100 mg/kg), ALA (100 mg/kg), and sham. Diazinon induced lipid peroxidation and oxidative stress and reduced the distribution and mRNA expression of laminin and fibronectin. ALA reduces the nephrotoxic effects of diazinon. In conclusion, ALA plays a therapeutic role in the treatment of oxidative stress-associated toxicities and diseases.

本研究旨在探究α-硫辛酸（alpha-lipoic acid, ALA）对暴露于二嗪农（diazinon, DZN）的雄性Wistar大鼠肾小体氧化应激指标，以及纤连蛋白（fibronectin）、层粘连蛋白（laminin）表达水平的影响。实验动物被划分为以下组别：对照组、二嗪农给药组（40 mg/kg）、二嗪农+α-硫辛酸联合给药组（40 mg/kg + 100 mg/kg）、α-硫辛酸给药组（100 mg/kg）以及假手术组。结果显示，二嗪农可诱导脂质过氧化与氧化应激，并降低层粘连蛋白与纤连蛋白的分布及其mRNA表达水平；α-硫辛酸可缓解二嗪农的肾毒性作用。综上，α-硫辛酸在氧化应激相关毒性反应与疾病的治疗中具备治疗作用。