Antifungal activity of components used for decontamination of dental prostheses on the growth of Candida albicans

Introduction:The effectiveness of antimicrobial solutions employed in dental prosthesis decontamination is still uncertain.Aim: To evaluate the antifungal activity of cleaners used in the decontamination of dental prostheses on the growth of Candida albicans.Material and method: The evaluated products were: Corega Tabs(r) (S1), Sodium Hypochlorite 1% (S2), Sodium Bicarbonate 1% (S3), Hydrogen Peroxide 1% (S4), Chlorhexidine Digluconate 0.12% - Periogard (r) (S5), Mouthrinse based on essential oils - Listerine(r) (S6), essential oil from Rosmarinus officinalis (rosemary) at concentrations of 1% (S7) and 2% (S8). The antifungal activity of the products was evaluated by agar diffusion technique and the determination of microbial death curve of samples of C. albicans (ATCC 90028) in concentration 1.5 × 106 CFU/mL. The tests were performed in triplicate and statistical analysis was made by ANOVA Two-Way and Tukey tests, with the confidence level of 95%.Result:The average of the zones of inhibition growth, in millimeters, obtained for the products were: 0.0 (S1), 44.7 (S2), 0.0 (S3), 21.6 (S4), 10.0 (S5), 6.1 (S6), 0.0 (S7) and 2.4 (S8). Considering the determination of microbial death curve, all products showed a statistical difference (p<0.01) from control (0.85% sodium chloride) and S3 groups. Fungal growth less than 2×104 CFU/mL and an accentuation of the microbial death curve were observed after 30 minutes, with exception for S3 and control groups.Conclusion:The studied compounds, with the exception of Sodium Bicarbonate, have antifungal effect against C. albicans, which contribute for dental prostheses hygiene.

引言：当前用于牙科义齿消毒的抗菌溶液的有效性仍未明确。研究目的：评估牙科义齿消毒所用清洁剂对白色念珠菌（Candida albicans）生长的抗真菌活性。材料与方法：本次评估的受试产品包括：Corega Tabs(r)（S1）、1%次氯酸钠（S2）、1%碳酸氢钠（S3）、1%过氧化氢（S4）、0.12%葡萄糖酸氯己定——Periogard(r)（S5）、精油型漱口水——Listerine(r)（S6）、浓度分别为1%（S7）与2%（S8）的迷迭香（Rosmarinus officinalis）精油。采用琼脂扩散法评估各产品的抗真菌活性，并测定浓度为1.5×10^6 CFU/mL的白色念珠菌（ATCC 90028）样本的微生物死亡曲线。所有实验均设置三次重复，统计学分析采用双因素方差分析（ANOVA Two-Way）与Tukey检验，置信水平设定为95%。结果：各受试产品对应的抑菌圈直径（单位：毫米）依次为：S1：0.0、S2：44.7、S3：0.0、S4：21.6、S5：10.0、S6：6.1、S7：0.0、S8：2.4。就微生物死亡曲线的测定结果而言，所有受试产品与对照组（0.85%氯化钠溶液）及S3组均存在统计学差异（p<0.01）。除S3组与对照组外，其余各组在30分钟后均观测到真菌菌落数降至2×10^4 CFU/mL以下，且微生物死亡曲线呈现显著陡降趋势。结论：本次研究涉及的各类化合物中，除碳酸氢钠外，其余均对白色念珠菌具有抗真菌活性，可为牙科义齿的卫生维护提供有效支撑。