Raw data for the role of acetyl phosphate in bypassing the cell membrane electrical potential sensor LytS

Data 1<br>Autophosphorylation of LytS.<br>Quantification of phosphorylated LytS bands by NIH Image J. Average of two trials. Data 2<br>Phosphorylation of LytR by acetyl phosphate.<br>Quantification of phosphorylated LytR bands by NIH ImageJ. Data 3<br>Phosphorylation of LytR-N by acetyl phosphate.<br>Quantification of phosphorylated LytR-N bands by NIH ImageJ. Data 4<br>Quantification of LytR bands in native-PAGEs (Figure 6A and B) by NIH Image J. LytR protein was phosphorylated by acetyl phosphate and its dephosphorylation by LytS was monitored by native-PAGE, whereby the phosphorylated dimer LytR becomes monomer after dephosphorylation. The column “corrected” represents the data after correcting for background signal at 0 min, and considering the band labeled “Monomer”, in the absence of LytS and ATP, as 100% unphosphorylated LytR.

数据集1：LytS的自磷酸化实验。采用NIH ImageJ对磷酸化LytS条带进行定量分析，结果为两次重复实验的平均值。
数据集2：乙酰磷酸介导的LytR磷酸化实验。采用NIH ImageJ对磷酸化LytR条带进行定量分析。
数据集3：乙酰磷酸介导的LytR-N磷酸化实验。采用NIH ImageJ对磷酸化LytR-N条带进行定量分析。
数据集4：采用NIH ImageJ对非变性聚丙烯酰胺凝胶电泳（native-PAGE）（图6A与图6B）中的LytR条带进行定量分析。本实验中，LytR蛋白经乙酰磷酸完成磷酸化，随后通过LytS介导的去磷酸化过程，采用非变性-PAGE进行监测：磷酸化的LytR二聚体在去磷酸化后会解离为单体。"校正后"列代表扣除0分钟时间点的背景信号后得到的数据，并将无LytS与ATP存在时标记为"单体"的条带定义为100%未磷酸化的LytR。