Table 4. Comparison of different screening methods.
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+++ = highest efficacy.aThe detection cost for 10000 mutants.bThese values are the lowest titers of surfactin that still occurred visual detection by the respective assays.cTo compare the size of transparent circles in blood agar, the average colonies on one agar plate should be limited in 10–20 (depends on the titers of surfactin). So it needs 500–1000 pieces of blood agar plates containing 10–20 L culture medium. It needs 500–1000 mL defibrinated sheep blood (5% blood in blood agar), which costs 75–150 US dollars.
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2015-12-02
