Table 4. Comparison of different screening methods.

+++ = highest efficacy.aThe detection cost for 10000 mutants.bThese values are the lowest titers of surfactin that still occurred visual detection by the respective assays.cTo compare the size of transparent circles in blood agar, the average colonies on one agar plate should be limited in 10–20 (depends on the titers of surfactin). So it needs 500–1000 pieces of blood agar plates containing 10–20 L culture medium. It needs 500–1000 mL defibrinated sheep blood (5% blood in blood agar), which costs 75–150 US dollars.+++ 代表最高效力。 a. 10000个突变体的检测成本。 b. 上述数值为可通过对应检测方法实现目视检出的最低表面活性素（surfactin）效价。 c. 为比对血平板（blood agar）上的透明圈尺寸，单个琼脂平板上的菌落数需控制在10~20范围内（具体取决于表面活性素的效价）。因此需制备500~1000块含10~20L培养基的血平板，同时需准备500~1000mL脱纤维羊血（血平板中血液添加比例为5%），采购成本为75~150美元。