Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes

Raw multichannel and/or Z-stack source data from time series images in TIF format to accompany publication of: <strong>Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes</strong> Jina Ko¹, Martin Wilkovitsch², Juhyun Oh¹, Rainer Kohler¹, Evangelia Bolli^1,3, Mikael J. Pittet^1,3,4,5, Claudio Vinegoni¹, David B. Sykes^6,7, Hannes Mikula², Ralph Weissleder^1,8*, Jonathan C. T. Carlson^1,7* ¹Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114 ² Institute of Applied Synthetic Chemistry, TU Wien, 1060 Vienna, Austria ³ Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland ⁴ Ludwig Institute for Cancer Research, Lausanne Branch, Switzerland ⁵ AGORA Cancer Center, Lausanne, Switzerland ⁶ Center for Regenerative Medicine, Massachusetts General Hospital, Boston, MA, USA ⁷Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA ⁸Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115本数据集为配套下述论文发表的TIF格式时序图像原始多通道及Z堆叠（Z-stack）源数据： **《基于荧光探针生物正交循环技术的活细胞与组织时空多重免疫荧光成像》** 作者：吉娜·科¹、马丁·威尔科维奇²、吴珠贤¹、莱纳·科勒¹、埃万耶利亚·博利^1,3、米凯尔·J·皮特特^1,3,4,5、克劳迪奥·维尼奥尼¹、戴维·B·赛克斯^6,7、汉内斯·米库拉²、拉尔夫·魏斯莱德^1,8*、乔纳森·C·T·卡尔森^1,7* ¹ 麻省总医院系统生物学中心，美国马萨诸塞州波士顿市剑桥街185号CPZN 5206室，邮编02114 ² 维也纳工业大学应用合成化学研究所，奥地利维也纳1060区 ³ 日内瓦大学病理与免疫学系，瑞士日内瓦 ⁴ 瑞士洛桑路德维希癌症研究所 ⁵ 瑞士洛桑AGORA癌症中心 ⁶ 麻省总医院再生医学中心，美国马萨诸塞州波士顿市 ⁷ 哈佛医学院麻省总医院内科系，美国马萨诸塞州波士顿市，邮编02114 ⁸ 哈佛医学院系统生物学系，美国马萨诸塞州波士顿市朗伍德大道200号，邮编02115