Data from: Effects of parasitic sex-ratio distorters on host genetic structure in the Armadillidium vulgare-Wolbachia association

In the pill bug Armadillidium vulgare (Crustacea, Oniscidea) Wolbachia facilitates its spread through vertical transmission via the eggs by inducing feminization of genetic males. The spread of feminizing Wolbachia within and across populations is therefore expected to influence mtDNA genetic structure by hitchhiking. To test this hypothesis, we analysed nuclear and mtDNA genetic structure, and Wolbachia prevalence in 13 populations of the pill bug host. Wolbachia prevalence (ranging from 0 to 100% of sampled females) was highly variable among populations. All three Wolbachia strains previously observed in A. vulgare were present (wVulC, wVulM and wVulP) with wVulC being the most prevalent (9 out of 13 populations). The host showed a genetic structure on five microsatellite loci that is compatible with isolation by distance. The strong genetic structure observed on host mtDNA was correlated to Wolbachia prevalence: three mitotypes were in strong linkage disequilibrium with the three strains of Wolbachia. Neutrality tests showed that the mtDNA polymorphism is not neutral and we thus suggest that this unusual pattern of mtDNA polymorphism found in A. vulgare was due to Wolbachia.

在卷球鼠妇（Armadillidium vulgare，甲壳纲等足目）中，沃尔巴克氏体（Wolbachia）可通过诱导遗传雄性雌性化，经虫卵实现垂直传播，从而促进自身扩散。因此，具雌性化效应的沃尔巴克氏体在种群内部及跨种群的扩散，有望通过遗传搭车效应影响线粒体DNA（mitochondrial DNA，简称mtDNA）的遗传结构。为验证该假说，我们对13个卷球鼠妇宿主种群的核基因与mtDNA遗传结构，以及沃尔巴克氏体感染率进行了分析。种群间沃尔巴克氏体感染率差异显著，抽样雌性个体的感染率范围为0至100%。本研究检出了此前在A. vulgare中报道的全部3种沃尔巴克氏体菌株（wVulC、wVulM与wVulP），其中wVulC最为普遍，在13个种群中有9个可检出该菌株。宿主在5个微卫星位点（microsatellite loci）上的遗传结构符合距离隔离模式（isolation by distance）。宿主mtDNA所呈现的显著遗传结构与沃尔巴克氏体感染率存在显著关联：3种线粒体单倍型分别与3种沃尔巴克氏体菌株呈现极强的连锁不平衡（linkage disequilibrium）。中性检验（neutrality tests）结果显示，该mtDNA多态性并非中性演化，因此我们推测，卷球鼠妇中这种非典型的mtDNA多态性模式正是由沃尔巴克氏体介导产生的。