MOESM4 of A Pseudomonas putida efflux pump acts on short-chain alcohols

Additional file 4. Quantification of extracellular n-butanol using GC-FID. The spreadsheet contains the estimated fraction of viable cells after incubation in PBS containing 0.2 and 1% n-butanol, and the measurement values of quantified extracellular n-butanol in induced (ttgABC + ) and non-induced strains (ttgABC - ). The original readings for n-butanol and the internal standard isoprenol are shown, as well as the n-butanol measurements normalized by the standard, and the derived concentrations as volume per volume and milligrams per milliliter. The inlay shows the n-butanol standard curve, indicating that concentrations are in a linear measurement range.补充文件4：采用气相色谱-火焰离子化检测器（GC-FID）对胞外正丁醇进行定量分析。本电子表格包含在含有0.2%和1%正丁醇的磷酸盐缓冲液（PBS，Phosphate Buffered Saline）中孵育后活细胞占比的估算值，以及诱导型（ttgABC+）与非诱导型（ttgABC-）菌株的胞外正丁醇定量检测结果。表格中同时列出了正丁醇与内标异戊烯醇的原始检测数据，以及经内标归一化后的正丁醇检测结果，还有换算得到的体积比浓度与毫克/毫升浓度。内嵌图展示了正丁醇标准曲线，表明本次检测浓度处于线性检测范围内。