Primers used for amplification of AT passenger domains.
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Forward primers begin with sequence containing attB1 sites (underlined) and additional nucleotides (gg) to place following sequence in-frame with polyhistine coding sequence in ultimate expression plasmid followed by gene-specific target sequences (…xxx-3′).Reverse primers begin with sequence containing attB2 sites (underlined) followed by stop codon (tta), then gene-specific target sequence.
创建时间：
2015-12-02
