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prp43 COLD SHIFT. Saccharomyces cerevisiae

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NIAID Data Ecosystem2026-03-06 收录
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Cold senstive alleles of prp43, S249A and G429A were assayed for splicing and RNA processing defects. Keywords: splicing, pre-mRNA processing Overall design: RNA was isolated from cold senstive alleles of prp43, S249A and G429A after cold shift to 16C for 60 mins. Cy3/Cy5 labeled RNA's were hybridized to Yeast Splicing Chip v62 using WT RNA cold shifted to 16C for 60 mins as a reference. There are 4 hybes in the series which include reciprocal dye swap experiements for each cold sensitive allele.

创建时间:
2005-10-20
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