Summary of data analysis of RNAseq of DMSO, MCT, LPS and MCT/LPS treated rats

Laboratory models of lung disease often apply a stimulus to a healthy animal, which does not really mimic the complexity of human disease. The model here describes that prior stimulus with a chemical (MCT) - known to elicit progressive cardiovascular changes - predisposes to altered lung responses to a subsequent pathogen stimulus (LPS). This offers a laboratory model that incorporates the biological history of an animal, which impacts how the lung responds to a pathogen.The results here are described in the manuscript 10.64898/2025.12.21.695829. The raw data and metadata have been successfully uploaded to NCBI. The Bioproject Accession is: PRJNA1383992.

传统肺部疾病实验模型通常仅向健康动物施加单一刺激，该造模方式难以真实模拟人类疾病的复杂病理过程。本研究构建的实验模型预先给予动物化学物质（MCT）——该物质可诱导进行性心血管病变，能够使动物在后续受到病原体刺激（LPS）时出现异常的肺部应答反应。该模型纳入了动物的既往生物学暴露史，可精准反映肺部对病原体的应答模式。本研究结果已刊载于论文10.64898/2025.12.21.695829。原始数据与元数据已成功上传至美国国家生物技术信息中心（NCBI），其生物项目登录号（Bioproject Accession）为：PRJNA1383992。