Metagenomic analysis of virome cross-talk between cultivated Solanum lycopersicum and wild Solanum nigrum

We selected tomato (Solanum lycopersicum) and european black nightshade (Solanum nigrum) as a pair of related crop-weed species to study the diversity and potential flow of viral populations using high throughput sequencing (HTS) based metagenomics. A total of 20 libraries were prepared and sequenced on an Illumina HiSeq 3000 system at the GenoToul platform (INRA Toulouse, France).

本研究选取番茄（Solanum lycopersicum）与欧洲龙葵（Solanum nigrum）这一组亲缘关系密切的作物-杂草物种对作为研究对象，采用基于高通量测序（High Throughput Sequencing, HTS）的宏基因组学技术，探究病毒种群的多样性及其潜在传播流。共计构建20个文库，并于法国图卢兹INRA的GenoToul平台上，使用Illumina HiSeq 3000测序系统完成测序。