Identification of HNF1A targets in WT hPSC-derived kidney organoids

To determine the transcriptional targets of HNF1A in human kidney cells, we performed chromatin immunoprecipitation sequencing on D28 kidney organoids generated from WT hPSCs using an established protocol by Morizane et al. (2015) doi: 10.1038/nbt.3392. ChIP-Seq analysis revealed that HNF1A preferentially bound onto the promoter regions of various genes that are predominantly expressed in renal proximal epithelial cells. qRT-PCR validation was performed on various human kidney cell models to confirm that HNF1A binds to promoter regions of selected target genes to regulate their gene expression. ChIP-Seq was performed on end-stage kidney organoids obtained on day 28 of the directed differentiation of WT hPSCs (1 clone from H9).