Inventory of soil prokaryotic and fungal microbiome (via 16S rRNA gene amplicons and ITS sequencing) from Shark River Slough and Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, February 2019 - October 2020

Global sea-level rise is transforming coastal ecosystems, especially freshwater wetlands, in part due to increased saltwater exposure, leading to change in soil microbial communities and many important biogeochemical processes. Given the high spatial and temporal heterogeneity in coastal wetlands, especially in tropical or subtropical climates characterized by seasonal temperature, precipitation, and tidal fluctuations, it remains unclear which environmental factors influence the compositions of soil microbial communities in wetlands affected by varying degrees of sea-water intrusion. To address this, a two-year survey was conducted on microbial community structure in submerged surface soils from 14 wetland sites across the Florida Everglades, representing three major ecosystem types, i.e. freshwater marshes, mangrove forests, and seagrass meadows. Bulk surface soil samples of each site were collected from February 2019 to October 2020 to cover dry and wet seasons. In addition to bulk soil samples, soil cores were collected from each site in August 2020 to assess vertical gradients of microbial communities. The dataset contains amplicon sequencing data of 16S rRNA gene (both bulk soil and soil cores) and ITS gene (only the bulk soil). The 2019 to 2020 data are published in Zhao et al. 2023. A detailed list of sequence data and their accession numbers in GenBank is provided, and data collection is complete. This data package is an inventory of sequence read archive (SRA) entries available through GenBank BioProject PRJNA804243 (https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA804243), PRJNA804246 (https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA804246), and PRJNA804228 (https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA804228). This data package is associated with the following publication: Zhao, J., Chakrabarti, S., Chambers, R., Weisenhorn, P., Travieso, R., Stumpf, S., Standen, E., Briceno, H., Troxler, T., Gaiser, E., Kominoski, J., Dhillon, B., & Martens-Habbena, W. (2023). Year-around survey and manipulation experiments reveal differential sensitivities of soil prokaryotic and fungal communities to saltwater intrusion in Florida Everglades wetlands. Science of The Total Environment, 858, 159865. https://doi.org/10.1016/j.scitotenv.2022.159865 Instead of citing this package, which is an inventory, please cite the original GenBank data or journal article, as appropriate. Citation guidance for the journal article is available on the respective publisher's website.

全球海平面上升正在改变沿海生态系统，尤以淡水湿地为甚，其部分诱因在于盐水暴露程度增加，进而导致土壤微生物群落结构与诸多关键生物地球化学过程发生改变。鉴于沿海湿地存在高度的空间与时间异质性，尤其是在以季节温度、降水及潮汐波动为特征的热带或亚热带气候湿地中，目前仍未明确哪些环境因子会影响受不同程度海水入侵影响的湿地土壤微生物群落组成。
为解决这一科学问题，研究团队对佛罗里达大沼泽地（Florida Everglades）14个湿地点位的淹没表层土壤开展了为期两年的微生物群落结构调查，这些点位涵盖三大典型生态系统类型，即淡水沼泽、红树林森林与海草床。2019年2月至2020年10月期间，采集各点位的混合表层土壤样品（bulk surface soil sample）以覆盖旱季与雨季；此外，研究团队于2020年8月从各点位采集土壤岩芯样品，用以评估微生物群落的垂直分布梯度特征。
本数据集包含16S rRNA基因（16S rRNA gene，涵盖混合表层土壤样品与土壤岩芯样品）以及ITS基因（ITS gene，仅针对混合表层土壤样品）的扩增子测序数据。2019至2020年的相关研究数据已发表于Zhao等人2023年的学术成果中。本研究提供了序列数据的详细清单及其在GenBank中的登录号，数据采集工作已全部完成。
本数据包为序列读取档案（Sequence Read Archive, SRA）条目的汇总清单，可通过GenBank生物项目（BioProject）PRJNA804243（https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA804243）、PRJNA804246（https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA804246）及PRJNA804228（https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA804228）获取。
本数据包与以下学术论文相关联：Zhao, J., Chakrabarti, S., Chambers, R., Weisenhorn, P., Travieso, R., Stumpf, S., Standen, E., Briceno, H., Troxler, T., Gaiser, E., Kominoski, J., Dhillon, B., & Martens-Habbena, W. (2023). 全年调查与操控实验揭示佛罗里达大沼泽地湿地中土壤原核生物与真菌群落对海水入侵的差异化敏感性. 《整体环境科学（Science of The Total Environment）》, 858, 159865. https://doi.org/10.1016/j.scitotenv.2022.159865
相较于引用本汇总数据包，请酌情引用原始GenBank数据或相关期刊论文。期刊论文的引用指南可查阅对应出版商的官方网站。