Upregulation of IL-10 expression inhibits the proliferation of human periodontal ligament stem cells

Abstract: The abnormal increase in proliferation rate of human periodontal ligament stem cells (PDLSCs) is considered to be involved in the pathogenesis of periodontitis, a disease in which the IL-10-mediated anti-inflammatory pathway plays a critical role. This study aimed to investigate the involvement of microRNA-466l in periodontitis and to explore the possible interaction between IL-10 and microRNA-466l. PDLSCs were obtained from periodontitis-affected teeth and healthy control teeth. The expression of microRNA-466l and IL-10 mRNA was measured in PDLSCs using RT-qPCR. The proliferation ability of PDLSCs was analyzed using CCK-8 assays. Overexpression of microRNA-466l in a PDLSC cell line was established using two different types of PDLSCs, and the effect of microRNA-466l overexpression on IL-10 expression and cell proliferation were detected by western blot and CCK-8 assays, respectively. We found that expression levels of microRNA-466l and IL-10 mRNA were significantly lower (P < 0.05) in PDLSCs derived from periodontitis-affected teeth compared to those derived from healthy teeth. However, the cell proliferation ability was significantly higher in the PDLSCs derived from periodontitis-affected teeth. Meanwhile microRNA-466l overexpression decreased cell proliferation rates of both types of PDLSCs and upregulated IL-10 expression. Together, these data suggest that microRNA-466l can upregulate IL-10 and reduce the proliferation rate of PDLSCs.

Abstract: 人类牙周膜干细胞（human periodontal ligament stem cells, PDLSCs）增殖速率异常升高被认为与牙周炎的发病机制相关，而白细胞介素10（IL-10）介导的抗炎通路在该病中发挥关键作用。本研究旨在探讨微小RNA（microRNA）-466l在牙周炎中的作用，并探究IL-10与microRNA-466l之间可能存在的相互作用。本研究从罹患牙周炎的患牙及健康对照牙中分离培养PDLSCs。采用逆转录实时定量聚合酶链反应（RT-qPCR）检测PDLSCs中microRNA-466l与IL-10 mRNA的表达水平；采用CCK-8法分析PDLSCs的增殖能力。通过两种不同来源的PDLSCs构建microRNA-466l过表达细胞模型，分别采用蛋白质印迹法（western blot）与CCK-8法检测microRNA-466l过表达对IL-10表达及细胞增殖的影响。研究结果显示，与健康牙来源的PDLSCs相比，牙周炎患牙来源的PDLSCs中microRNA-466l与IL-10 mRNA的表达水平显著降低（P < 0.05），但该类细胞的增殖能力显著升高。同时，microRNA-466l过表达可降低两种PDLSCs的增殖速率，并上调IL-10的表达。综上，本研究结果表明microRNA-466l可上调IL-10的表达并降低PDLSCs的增殖速率。