TExTra Test Data and Tool Dependencies
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The dataset is intended for demonstration, testing, and validation purposes only, and enables users to verify the correct installation and execution of the TExTra workflow. It is designed to work seamlessly with the example scripts and default parameters provided in the TExTra documentation. The dataset includes: Paired-end RNA-seq BAM files representing simulated mouse heart developmental samples. Reverse-stranded (rf) library layout, suitable for strandedness-specific analyses. Reference genome annotation (GTF): Gencode vM21 primary assembly annotations. Transposable element (TE) annotation (GTF): RepeatMasker-based TE annotations for mouse. Reference genome FASTA file: GRCm38 primary assembly. Tool packages: TACO v0.7.3 (taco-v0.7.3.Linux_x86_64.tar.gz) for transcriptome assembly and merging. PLEK v2 (PLEKv2_allfiles_240807.tar.gz) for lncRNA prediction. Note: TACO and PLEK can be downloaded directly and used after extraction in the util/ directory without requiring additional downloads. All files are ready to use and compatible with the default workflows and example pipelines described in the TExTra documentation, allowing users to quickly test and validate the software installation and computational workflow.
本数据集仅用于演示、测试与验证用途,旨在帮助用户验证TExTra工作流的正确安装与运行,可与TExTra文档中提供的示例脚本及默认参数无缝适配。 本数据集包含: 双端RNA测序BAM文件:代表模拟的小鼠心脏发育样本。 反向链(rf)文库布局,适用于链特异性分析。 参考基因组注释文件(GTF):Gencode vM21 主要组装注释。 转座因子(Transposable Element, TE)注释文件(GTF):基于RepeatMasker的小鼠转座因子注释。 参考基因组FASTA文件:GRCm38 主要组装版本。 工具包: TACO v0.7.3(taco-v0.7.3.Linux_x86_64.tar.gz:用于转录组组装与合并。 PLEK v2(PLEKv2_allfiles_240807.tar.gz:用于长链非编码RNA(lncRNA)预测。 注意:TACO与PLEK可直接下载,解压至util/目录后即可使用,无需额外下载。 所有文件均可直接使用,与TExTra文档中描述的默认工作流及示例流程兼容,可帮助用户快速测试并验证软件安装与计算工作流。



