SH1044781.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE提供了一套统一的解决方案，用于界定、识别、交流以及处理基于DNA的物种假说（Species Hypotheses，以下简称SH）。国际核苷酸序列数据库中的所有真菌ITS（Internal Transcribed Spacer）序列，通过应用一系列动态距离阈值（0.5%~3.0%），被聚类至近似物种水平。所有物种假说均会被赋予一个以DOI（Digital Object Identifier，数字对象标识符）形式存在的唯一且稳定的名称，其分类学与生态学注释将通过分布式的、基于网页的第三方注释工作完成核验。SH将尽可能关联到对应分类单元的名称及其分类层级（如门、纲、目等），关联依据为该SH下所有序列的鉴定结果。将选取一条自动或人工指定的序列，作为每个此类SH的代表序列。上述代表序列已发布于https://unite.ut.ee/repository.php，可供科学界用于本地序列相似性搜索、下一代测序（next-generation sequencing）分析流程等科研场景。随着公共真菌ITS序列库规模的增长，该系统及其配套数据将自动完成更新。