Photocaged 5‑(Hydroxymethyl)pyrimidine Nucleoside Phosphoramidites for Specific Photoactivatable Epigenetic Labeling of DNA
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https://figshare.com/articles/dataset/Photocaged_5_Hydroxymethyl_pyrimidine_Nucleoside_Phosphoramidites_for_Specific_Photoactivatable_Epigenetic_Labeling_of_DNA/13201076
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资源简介：
5-Hydroxymethylcytosine and uracil
are epigenetic nucleobases, but their biological roles are still unclear.
We present the synthesis of 2-nitrobenzyl photocaged 5-hydroxymethyl-2′-deoxycytidine
and uridine 3′-O-phosphoramidites and their
use in automated solid-phase synthesis of oligonucleotides (ONs) modified
at specific positions. The ONs were used as primers for PCR to construct
DNA templates modified in the promoter region that allowed switching
of transcription through photochemical uncaging.
创建时间：
2020-11-06
