Identification of Wnt target genes associated with malignant phenotypes in CTNNB1-mutated NSCLC

Microarray analysis comparing the expression profiles of siRNA-mediated CTNNB1-silenced and CTNNB1-retained HCC15 cells harboring a homozygous CTNNB1 S45F mutation revealed that besides several known Wnt genes, LGR6 was significantly downregulated by CTNNB1 knockdown in HCC15 cells. Gene expression was examined in siRNA-mediated CTNNB1-silenced HCC15 cells and CTNNB1-retained (control siRNA-treated) HCC15 cells. HCC15 cells were transfected with an siRNA targeting CTNNB1 and were collected at 48 h post-transfection, followed by tRNA extraction from the cells.