The critical value for the CLS test (alpha = 0.05) and the number of total mutations needed to achieve 80% power.

Table 1 Caption: Computations based on an exact binominal distribution under reasonable assumptions on p0, the background rate of called shared mutations in independent tumor pairs, and pa, the expected rate of called shared mutations in clonal tumor pairs. Under recommended assumptions, at least 44 called HC mutations are needed to implement the CLS test. The appropriate values of p0 and pa will depend on the sequencing technology and tumor type and should be chosen prior to conducting the CLS test. The CLS test rejects the hypothesis of independence in favor of clonal status if CLS > c/n,The critical value for the CLS test (alpha = 0.05) and the number of total mutations needed to achieve 80% power.