The critical value for the CLS test (alpha = 0.05) and the number of total mutations needed to achieve 80% power.

Table 1 Caption: Computations based on an exact binominal distribution under reasonable assumptions on p0, the background rate of called shared mutations in independent tumor pairs, and pa, the expected rate of called shared mutations in clonal tumor pairs. Under recommended assumptions, at least 44 called HC mutations are needed to implement the CLS test. The appropriate values of p0 and pa will depend on the sequencing technology and tumor type and should be chosen prior to conducting the CLS test. The CLS test rejects the hypothesis of independence in favor of clonal status if CLS > c/n,The critical value for the CLS test (alpha = 0.05) and the number of total mutations needed to achieve 80% power.表1 说明：基于针对p₀（独立肿瘤对中被检出的共有突变的背景发生率）与pₐ（克隆性肿瘤对中被检出的共有突变的预期发生率）的合理假设，基于精确二项分布完成的计算。按照推荐假设实施CLS检验时，至少需要44个经高置信度（High Confidence, HC）标注的突变。p₀与pₐ的适宜取值取决于测序技术与肿瘤类型，且需在开展CLS检验前确定。若CLS > c/n，则CLS检验将拒绝独立性假设，转而支持肿瘤呈克隆性的结论。本说明同时给出了显著性水平α=0.05时CLS检验的临界值，以及达到80%检验效力所需的总突变数。